CSIR-CSIO
Analytical Instrument Facility

Fluorescence Spectrophotometer

Instruments Details
Specifications:
Make & Model
:Cary-Eclipse ,Varian FL1007M000
Wavelength range
:190‒900 nm wavelength
Scan rate
:0.01‒24,000 nm/min
Xe pulsed lamp source (80 Hz)
Czerny-Turner monochromators with 12.5 cm focal width
12.5 cm focal width
Fixed selective SWE from 1.5‒20 nm
Working Principles

Fluorescence spectroscopy is a type of electromagnetic spectroscopy that analyzes fluorescence emission from a sample. It involves using a beam of light, usually ultraviolet light, that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily, visible light.

In fluorescence, the species is first excited, by absorbing a photon, from its ground electronic state to one of the various vibrational states in the excited electronic state. Collisions with other molecules cause the excited molecule to lose vibrational energy until it reaches the lowest vibrational state of the excited electronic state. This process is often visualized with a Jablonski diagram.

Applications

It has measurement modes for fluorescence, chemiluminescence, bioluminescence, and phosphorescence for following sample.

  • Single liquid sample using quartz cuvette
  • Multiple liquid samples using 96-well plate reader
  • Solid film sample

Contact Person
Dr. Sachin Tyagi
Contact: +91-172-2642545
Email Id : sachintyagi.iitr@gmail.com
Charges includes GST

Single liquid sample using quartz cuvette and Solid film sampleRs. 350per sample


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